Journal:

Article Title: Thrombospondin-1 Regulates the Normal Prostate In Vivo Through Angiogenesis and TGF-? Activation

doi: 10.1038/labinvest.2010.90

Figure Lengend Snippet: TSP-1 expression increases following androgen ablation in the mouse prostate and in patient-matched biopsy specimens in vivo. Prostate tissue sections were immunostained with anti-TSP antibody. (a) Prostate tissues were collected from intact or castrated (1-21 days post-castration; n=5 per group) wildtype (C57Bl/6) mice. Sections from intact and 3 days post-castration are shown. (b) TSP-1 staining intensity was graded on a 1-4 scale in the epithelium and stroma of the dorsal prostate. (c) The vessels were counted in the dorsal prostate stroma and the MVD:TSP-1 staining ratio calculated. (d) Patient-matched biopsies were taken from PCa patients before and 3-7 days after androgen ablation therapy. Six of eight patient tissues showed increased immunoreactivity following androgen ablation therapy. Pre- and post-therapy tissues are shown for two patients. (e) TSP-1 staining was graded on a 1-4 scale in the epithelial and stromal compartments. *Significantly increased in castrated compared to intact, P<0.012, and **significantly increased in human post-ADT tissues, P<00.013.

Article Snippet: Human Prostate Tissues Human prostate biopsy specimens were taken before and 3-7 days after surgical castration from PCa patients (n=8) at Umea University (Umea, Sweden).

Techniques: Expressing, In Vivo, Staining

Journal: Scientific Reports

Article Title: Derivation of human primary prostate epithelial cell lines by differentially targeting the CDKN2A locus along with expression of hTERT

doi: 10.1038/s41598-024-71306-5

Figure Lengend Snippet: RNA-Seq Analysis showing gene expression and clustering of the immortalized cell lines and PrECs. ( a ) Principal Component Analysis (PCA) of the RNA-Seq data for all the clones and PrEC. ( b ) Prostate tissue UMAP plots obtained using scRNA-Seq by the Human Protein Atlas (with superimposed expression of Actin (ACTG1)) identify all distinct populations in the primary human prostate . ( c ) Gene expression heat map of predicted correlation scores (Preds) of each clone and hPrECs to the cell populations identified in the human prostate using scRNA-Seq. Prediction scores range from 0.0 to 1.0 (blue to dark red scale on the left). The distinct cell populations identified by the Human Atlas using single cell-RNA-Seq are labeled by color and a number (top of the heatmap and legend on the right). Clones and PrEC RNA-Seq samples were analyzed in triplicate. The prediction score in B indicate that the clones are most closely related to the basal prostatic population designated c2 (labeled red/orange in the first column of the heat map).

Article Snippet: Fig. 6 RNA-Seq Analysis showing gene expression and clustering of the immortalized cell lines and PrECs. ( a ) Principal Component Analysis (PCA) of the RNA-Seq data for all the clones and PrEC. ( b ) Prostate tissue UMAP plots obtained using scRNA-Seq by the Human Protein Atlas (with superimposed expression of Actin (ACTG1)) identify all distinct populations in the primary human prostate . ( c ) Gene expression heat map of predicted correlation scores (Preds) of each clone and hPrECs to the cell populations identified in the human prostate using scRNA-Seq.

Techniques: RNA Sequencing, Gene Expression, Clone Assay, Expressing, Labeling